mouse soluble rankl Search Results


murine specific rankl elisa kit  (Boster Bio)
93
Boster Bio murine specific rankl elisa kit
Murine Specific Rankl Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/murine specific rankl elisa kit/product/Boster Bio
Average 93 stars, based on 1 article reviews
murine specific rankl elisa kit - by Bioz Stars, 2026-05
93/100 stars
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rankl cat. 315-11-100  (PeproTech)
90
PeproTech rankl cat. 315-11-100
Rankl Cat. 315 11 100, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rankl cat. 315-11-100/product/PeproTech
Average 90 stars, based on 1 article reviews
rankl cat. 315-11-100 - by Bioz Stars, 2026-05
90/100 stars
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mouse soluble rankl  (PeproTech)
90
PeproTech mouse soluble rankl
a-d. CD14 + human placental macrophages were differentiated into multinucleated giant cells (MGCs) in culture. Both CD14 + macrophages and MGCs were subjected to high-throughput RNA sequencing (RNA-seq). n = 6 biological replicates per group. Data source: GSE38747. a. Heatmap of differentially expressed genes between CD14 + macrophages and MGCs. b. Volcano plot of genes upregulated (red) or downregulated (blue) in MGCs relative to CD14 + macrophages. Cut-off values: |log 2 (fold change)| > 1 and P value < 0.001. c. Relative expression levels of MALAT1 and osteoclast markers were quantitated from the RNA-seq results. d. Gene set enrichment analysis (GSEA) of the RNA-seq data, showing the top 14 Gene Ontology (GO) pathways of 486 significant pathways. e-h. qPCR of Nfatc1 ( e ), Ctsk ( f ), Trap5 ( g ), and Malat1 ( h ) in RAW264.7 cells treated with <t>soluble</t> <t>RANKL</t> (50 ng/mL) for the indicated times. i. Schematic representation of the treatments used to evaluate the osteoclastogenic activity of RANKL, LPS, and TNF-α, with or without pretreatment (priming) with RANKL. j. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or LPS, with or without pretreatment with RANKL. k. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in j . l. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or TNF-α, with or without pretreatment with RANKL. m. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in l . Statistical significance in c and j-m was determined by a two-tailed unpaired t -test. Error bars are s.e.m.
Mouse Soluble Rankl, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse soluble rankl/product/PeproTech
Average 90 stars, based on 1 article reviews
mouse soluble rankl - by Bioz Stars, 2026-05
90/100 stars
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mouse trance (rankl) (soluble) recombinant protein  (PeproTech)
90
PeproTech mouse trance (rankl) (soluble) recombinant protein
a-d. CD14 + human placental macrophages were differentiated into multinucleated giant cells (MGCs) in culture. Both CD14 + macrophages and MGCs were subjected to high-throughput RNA sequencing (RNA-seq). n = 6 biological replicates per group. Data source: GSE38747. a. Heatmap of differentially expressed genes between CD14 + macrophages and MGCs. b. Volcano plot of genes upregulated (red) or downregulated (blue) in MGCs relative to CD14 + macrophages. Cut-off values: |log 2 (fold change)| > 1 and P value < 0.001. c. Relative expression levels of MALAT1 and osteoclast markers were quantitated from the RNA-seq results. d. Gene set enrichment analysis (GSEA) of the RNA-seq data, showing the top 14 Gene Ontology (GO) pathways of 486 significant pathways. e-h. qPCR of Nfatc1 ( e ), Ctsk ( f ), Trap5 ( g ), and Malat1 ( h ) in RAW264.7 cells treated with <t>soluble</t> <t>RANKL</t> (50 ng/mL) for the indicated times. i. Schematic representation of the treatments used to evaluate the osteoclastogenic activity of RANKL, LPS, and TNF-α, with or without pretreatment (priming) with RANKL. j. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or LPS, with or without pretreatment with RANKL. k. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in j . l. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or TNF-α, with or without pretreatment with RANKL. m. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in l . Statistical significance in c and j-m was determined by a two-tailed unpaired t -test. Error bars are s.e.m.
Mouse Trance (Rankl) (Soluble) Recombinant Protein, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse trance (rankl) (soluble) recombinant protein/product/PeproTech
Average 90 stars, based on 1 article reviews
mouse trance (rankl) (soluble) recombinant protein - by Bioz Stars, 2026-05
90/100 stars
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human / mouse rankl, soluble recombinant protein ddddk tag lyophilized  (Innovative Research Inc)
N/A
Human / Mouse RANKL, Soluble Recombinant Protein DDDDK Tag Lyophilized from Innovative Research has been recombinantly produced in HEK293 cells. This is a Lyophilized protein buffered in Contains PBS. Reconstitute with 100 and#956;l sterile water.
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a-d. CD14 + human placental macrophages were differentiated into multinucleated giant cells (MGCs) in culture. Both CD14 + macrophages and MGCs were subjected to high-throughput RNA sequencing (RNA-seq). n = 6 biological replicates per group. Data source: GSE38747. a. Heatmap of differentially expressed genes between CD14 + macrophages and MGCs. b. Volcano plot of genes upregulated (red) or downregulated (blue) in MGCs relative to CD14 + macrophages. Cut-off values: |log 2 (fold change)| > 1 and P value < 0.001. c. Relative expression levels of MALAT1 and osteoclast markers were quantitated from the RNA-seq results. d. Gene set enrichment analysis (GSEA) of the RNA-seq data, showing the top 14 Gene Ontology (GO) pathways of 486 significant pathways. e-h. qPCR of Nfatc1 ( e ), Ctsk ( f ), Trap5 ( g ), and Malat1 ( h ) in RAW264.7 cells treated with soluble RANKL (50 ng/mL) for the indicated times. i. Schematic representation of the treatments used to evaluate the osteoclastogenic activity of RANKL, LPS, and TNF-α, with or without pretreatment (priming) with RANKL. j. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or LPS, with or without pretreatment with RANKL. k. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in j . l. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or TNF-α, with or without pretreatment with RANKL. m. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in l . Statistical significance in c and j-m was determined by a two-tailed unpaired t -test. Error bars are s.e.m.

Journal: Research Square

Article Title: Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis

doi: 10.21203/rs.3.rs-2405644/v1

Figure Lengend Snippet: a-d. CD14 + human placental macrophages were differentiated into multinucleated giant cells (MGCs) in culture. Both CD14 + macrophages and MGCs were subjected to high-throughput RNA sequencing (RNA-seq). n = 6 biological replicates per group. Data source: GSE38747. a. Heatmap of differentially expressed genes between CD14 + macrophages and MGCs. b. Volcano plot of genes upregulated (red) or downregulated (blue) in MGCs relative to CD14 + macrophages. Cut-off values: |log 2 (fold change)| > 1 and P value < 0.001. c. Relative expression levels of MALAT1 and osteoclast markers were quantitated from the RNA-seq results. d. Gene set enrichment analysis (GSEA) of the RNA-seq data, showing the top 14 Gene Ontology (GO) pathways of 486 significant pathways. e-h. qPCR of Nfatc1 ( e ), Ctsk ( f ), Trap5 ( g ), and Malat1 ( h ) in RAW264.7 cells treated with soluble RANKL (50 ng/mL) for the indicated times. i. Schematic representation of the treatments used to evaluate the osteoclastogenic activity of RANKL, LPS, and TNF-α, with or without pretreatment (priming) with RANKL. j. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or LPS, with or without pretreatment with RANKL. k. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in j . l. TRAP staining images (left panel) and quantification (right panel) of RAW264.7 cells treated with RANKL or TNF-α, with or without pretreatment with RANKL. m. qPCR of Nfatc1 , Ctsk , and Malat1in the cells described in l . Statistical significance in c and j-m was determined by a two-tailed unpaired t -test. Error bars are s.e.m.

Article Snippet: Cells were seeded in 24-well or 6-well plates at a density of 2 × 10 4 or 5 × 10 5 cells per well; 50 ng/mL of mouse soluble RANKL (Peprotech, 315-11) was used to induce differentiation, and the culture medium was changed every 2 days.

Techniques: High Throughput Screening Assay, RNA Sequencing, Expressing, Activity Assay, Staining, Two Tailed Test